Fast chemiluminescent measurement of RNA polymerase activity based on photon counting technology.
نویسندگان
چکیده
A fast and simple assay for T7 RNA polymerase based upon chemiluminescent detection of the synthesized, digoxigenin-labeled RNA on a nylon membrane with anti-digoxigenin coupled alkaline phosphatase and CSPD as substrate is described. Activity of RNA polymerase is determined with high sensitivity by quantifying the emitted light of the microplate-formatted dot-blot membrane with a photon counting microplate luminometer and a specially designed filter adapter. The described method is one example for the application of this new adapter to measure luminescent membrane filters.
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ورودعنوان ژورنال:
- BioTechniques
دوره 15 5 شماره
صفحات -
تاریخ انتشار 1993